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mil 1r1 fc  (R&D Systems)


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    R&D Systems mil 1r1 fc
    Mil 1r1 Fc, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/il+1r1/Recombinant+Mouse+IL-1+RI+Fc+Chimera+Protein%2C+CF/pm41723527-109-41-42
    Average 94 stars, based on 5 article reviews
    mil 1r1 fc - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Staining:

    Article Title: Regulation of PD-L1 expression in non-small cell lung cancer by interleukin-1β.
    Article Snippet: .. Cells were stained with antibodies to CD326 (BioLegend, #324306), CD274 (BioLegend, #329708), IL-1R1 (R&D Systems, #FAB269P), IFNGR1 (R&D Systems, #FAB673P), IFNGR2 (R&D Systems, #FAB773A), CD11b (eBioscience, #11-0118-42), allophycocyanin-conjugated mouse IgG2b isotype control (BioLegend, #400322), phycoerythrin-conjugated goat IgG isotype control (BioLegend, #403004), phycoerythrin-conjugated mouse IgG1 isotype control (BioLegend, #400114), allophycocyaninconjugated goat IgG isotype control (R&D Systems, #IC108A), or fluorescein isothiocyanate-conjugated mouse IgG1 isotype control (BioLegend #400109). .. For the functional analysis of PBMCs cocultured with tumor cells, PBMCs were stained with antibody to CD3 (BioLegend, #344833), CD8 (BioLegend, #344711), CD4 (BioLegend, #564420), PD-1 (BioLegend, #329905), or Tim3 (BioLeged, #345011).

    Article Title: IL-1β Signaling modulates T follicular helper and regulatory cells in human lymphoid tissues.
    Article Snippet: .. The cells were then 499 stained for viability using 405/420 Viobility (1/500, Miltenyi), washed and then stained 500 with the following anti-human antibodies: CD3 (PerCP-Vio700, 1/100, Miltenyi, Cat# 501 130-109-465), ICOS (Vioblue, 1/100, Miltenyi, Cat# 130-100-737), CXCR5 (PE-502 Vio770, 1/500, Miltenyi, Cat# 130-117-358), CD4 (APC-Vio770, 1/500, Miltenyi, Cat# 503 130-113-223), CD38 (BV711, 1/400, BioLegend, Cat# 303527), CD25 (APC, 1/160, 504 BioLegend, Cat# 302610), HLA-DR (BV785, 1/20, BD Biosciences, Cat# 564041), 505 CD19 (PE-CF594, 1/200, BD Biosciences, Cat# 562294), IL-1R1 (PE, 1/80, R&D 506 Systems, Cat# FAB269P), IL-1R2 (AF700, 1/80, R&D Systems, Cat# FAB663N), PD-507 1 (PE-Cy5.5, 1/320, Beckman Coulter, Cat# B36123). ..

    Article Title: IL-1 β signaling modulates T follicular helper and regulatory cells in human lymphoid tissues
    Article Snippet: .. The cells were then stained for viability using 405/420 Viobility (1/500, Miltenyi), washed, and stained with the following anti-human antibodies: CD3 (PerCP-Vio700, 1/100, Miltenyi, Cat# 130-109-465), ICOS (Vioblue, 1/100, Miltenyi, Cat# 130-100-737), CXCR5 (PE-Vio770, 1/500, Miltenyi, Cat# 130-117-358), CD4 (APC-Vio770, 1/500, Miltenyi, Cat# 130-113-223), CD38 (BV711, 1/400, BioLegend, Cat# 303527), CD25 (APC, 1/160, BioLegend, Cat# 302610), HLA-DR (BV785, 1/20, BD Biosciences, Cat# 564041), CD19 (PE-CF594, 1/200, BD Biosciences, Cat# 562294), IL-1R1 (PE, 1/80, R&D Systems, Cat# FAB269P), IL-1R2 (AF700, 1/80, R&D Systems, Cat# FAB663N), and PD-1 (PE-Cy5.5, 1/320, Beckman Coulter, Cat# B36123 ). ..

    Article Title: Studies of Structure–Activity Relationship of 2-(Pyrrolidin-1ylmethyl)-1<i>H</i>-pyrrole-Based ST2 Inhibitors and Their Inhibition of Mast Cells Activation
    Article Snippet: .. The cells were then stained with the ST2, IL-1R1, isotype Abs expression (R&D system, ST2#FAB5231P, IL1R1#FAB269P, isotype#IC108P). ..

    Article Title: IL-1β Signaling modulates T follicular helper and regulatory cells in human lymphoid tissues.
    Article Snippet: The cells were then fixed using the BD Pharmingen 526 Transcription Factor kit. .. Intracellular staining was performed after fixation and 527 permeabilization (eBioscience Foxp3 Transcription factor staining buffer set, 528 Invitrogen), according to manufacturer's instructions, with the following anti-human 529 antibodies: Ki67 (BUV395, 1/160, BD Biosciences, Cat# 564071), Foxp3 (AF488, 530 1/20, BD Biosciences, Cat# 560047), CD38 (BV711, 1/400, BioLegend, Cat# 303527), 531 CD19 (PE-CF594, 1/200, BD Biosciences, Cat# 562294), IL-1R1 (PE, 1/80, R&D 532 Systems, Cat# FAB269P), IL-1R2 (AF700, 1/80, R&D Systems, Cat# FAB663N), PD1 533 (PE-Cy5.5, 1/320, Beckman Coulter, Cat# B36123), IL-4 (PerCP-Cy5.5, 1/160, BD 534 Biosciences, Cat# 561234), IL-21 (APC, 1/200, Miltneyi, Cat# 130-117-422), IL-10 535 (BV421, 1/160, Biolegend, Cat# 501422), IL-2 (BV605, 1/160, Biolegend, Cat# 536 500332). .. The cells were incubated for 40 minutes at 4°C, washed with the washing 537 buffer, resuspended in 1X PBS, and analyzed using a CytoFLEX LX cytometer 538 (Beckman Coulter).

    Article Title: IL-1β Signaling modulates T follicular helper and regulatory cells in human lymphoid tissues.
    Article Snippet: .. 552 Phenotypic profiling of cultured tonsillar whole cell suspensions 553 Both unstimulated cells (as controls) and cultured whole-cell suspensions were then 554 stained for viability using 405/420 Viobility (1/500, Miltenyi), washed and then stained 555 with the following anti-human antibodies: ICOS (Vioblue, 1/100, Miltenyi, Cat# 130-556 100-737), CD3 (PerCP-Vio700, 1/100, Miltenyi, Cat# 130-109-465), CXCR5 (PE-557 Vio770, 1/500, Miltenyi, Cat# 130-117-358), CD19 (APC, 1/500, Miltenyi, Cat# 130-558 110-250), CD4 (APC-Vio770, 1/500, Miltenyi, Cat# 130-113-223), HLA-DR (BV785, 559 1/20, BD Biosciences, Cat# 564041), IL-1R1 (PE, 1/80, R&D Systems, Cat# 560 FAB269P), IL-1R2 (AF700, 1/80, R&D Systems, Cat# FAB663N), CTLA-4 (BV605, 561 1/40, BioLegend, Cat# 369610), PD-1 (PE-Cy5.5, 1/320, Beckman Coulter, Cat# 562 B36123). ..

    Article Title: IL-1 β signaling modulates T follicular helper and regulatory cells in human lymphoid tissues
    Article Snippet: The cells were then fixed using the BD Pharmingen Transcription Factor kit. .. Intracellular staining was performed after fixation and permeabilization (eBioscience Foxp3 Transcription factor staining buffer set, Invitrogen), according to manufacturer’s instructions, with the following anti-human antibodies: Ki67 (BUV395, 1/160, BD Biosciences, Cat# 564071), Foxp3 (AF488, 1/20, BD Biosciences, Cat# 560047), CD38 (BV711, 1/400, BioLegend, Cat# 303527), CD19 (PE-CF594, 1/200, BD Biosciences, Cat# 562294), IL-1R1 (PE, 1/80, R&D Systems, Cat# FAB269P), IL-1R2 (AF700, 1/80, R&D Systems, Cat# FAB663N), PD1 (PE-Cy5.5, 1/320, Beckman Coulter, Cat# B36123 ), IL-4 (PerCP-Cy5.5, 1/160, BD Biosciences, Cat# 561234), IL-21 (APC, 1/200, Miltneyi, Cat# 130-117-422), IL-10 (BV421, 1/160, Biolegend, Cat# 501422), and IL-2 (BV605, 1/160, Biolegend, Cat# 500332). .. The cells were incubated for 40 minutes at 4°C, washed with the washing buffer, resuspended in 1× PBS, and analyzed using a CytoFLEX LX cytometer (Beckman Coulter).

    Article Title: IL-1 β signaling modulates T follicular helper and regulatory cells in human lymphoid tissues
    Article Snippet: .. Both unstimulated cells (as controls) and cultured whole-cell suspensions were then stained for viability using 405/420 Viobility (1/500, Miltenyi), washed, and stained with the following anti-human antibodies: ICOS (Vioblue, 1/100, Miltenyi, Cat# 130-100-737), CD3 (PerCP-Vio700, 1/100, Miltenyi, Cat# 130-109-465), CXCR5 (PE-Vio770, 1/500, Miltenyi, Cat# 130-117-358), CD19 (APC, 1/500, Miltenyi, Cat# 130-110-250), CD4 (APC-Vio770, 1/500, Miltenyi, Cat# 130-113-223), HLA-DR (BV785, 1/20, BD Biosciences, Cat# 564041), IL-1R1 (PE, 1/80, R&D Systems, Cat# FAB269P), IL-1R2 (AF700, 1/80, R&D Systems, Cat# FAB663N), CTLA-4 (BV605, 1/40, BioLegend, Cat# 369610), and PD-1 (PE-Cy5.5, 1/320, Beckman Coulter, Cat# B36123 ). ..

    Control:

    Article Title: Regulation of PD-L1 expression in non-small cell lung cancer by interleukin-1β.
    Article Snippet: .. Cells were stained with antibodies to CD326 (BioLegend, #324306), CD274 (BioLegend, #329708), IL-1R1 (R&D Systems, #FAB269P), IFNGR1 (R&D Systems, #FAB673P), IFNGR2 (R&D Systems, #FAB773A), CD11b (eBioscience, #11-0118-42), allophycocyanin-conjugated mouse IgG2b isotype control (BioLegend, #400322), phycoerythrin-conjugated goat IgG isotype control (BioLegend, #403004), phycoerythrin-conjugated mouse IgG1 isotype control (BioLegend, #400114), allophycocyaninconjugated goat IgG isotype control (R&D Systems, #IC108A), or fluorescein isothiocyanate-conjugated mouse IgG1 isotype control (BioLegend #400109). .. For the functional analysis of PBMCs cocultured with tumor cells, PBMCs were stained with antibody to CD3 (BioLegend, #344833), CD8 (BioLegend, #344711), CD4 (BioLegend, #564420), PD-1 (BioLegend, #329905), or Tim3 (BioLeged, #345011).

    Expressing:

    Article Title: Studies of Structure–Activity Relationship of 2-(Pyrrolidin-1ylmethyl)-1<i>H</i>-pyrrole-Based ST2 Inhibitors and Their Inhibition of Mast Cells Activation
    Article Snippet: .. The cells were then stained with the ST2, IL-1R1, isotype Abs expression (R&D system, ST2#FAB5231P, IL1R1#FAB269P, isotype#IC108P). ..

    Cell Culture:

    Article Title: IL-1β Signaling modulates T follicular helper and regulatory cells in human lymphoid tissues.
    Article Snippet: .. 552 Phenotypic profiling of cultured tonsillar whole cell suspensions 553 Both unstimulated cells (as controls) and cultured whole-cell suspensions were then 554 stained for viability using 405/420 Viobility (1/500, Miltenyi), washed and then stained 555 with the following anti-human antibodies: ICOS (Vioblue, 1/100, Miltenyi, Cat# 130-556 100-737), CD3 (PerCP-Vio700, 1/100, Miltenyi, Cat# 130-109-465), CXCR5 (PE-557 Vio770, 1/500, Miltenyi, Cat# 130-117-358), CD19 (APC, 1/500, Miltenyi, Cat# 130-558 110-250), CD4 (APC-Vio770, 1/500, Miltenyi, Cat# 130-113-223), HLA-DR (BV785, 559 1/20, BD Biosciences, Cat# 564041), IL-1R1 (PE, 1/80, R&D Systems, Cat# 560 FAB269P), IL-1R2 (AF700, 1/80, R&D Systems, Cat# FAB663N), CTLA-4 (BV605, 561 1/40, BioLegend, Cat# 369610), PD-1 (PE-Cy5.5, 1/320, Beckman Coulter, Cat# 562 B36123). ..

    Article Title: IL-1 β signaling modulates T follicular helper and regulatory cells in human lymphoid tissues
    Article Snippet: .. Both unstimulated cells (as controls) and cultured whole-cell suspensions were then stained for viability using 405/420 Viobility (1/500, Miltenyi), washed, and stained with the following anti-human antibodies: ICOS (Vioblue, 1/100, Miltenyi, Cat# 130-100-737), CD3 (PerCP-Vio700, 1/100, Miltenyi, Cat# 130-109-465), CXCR5 (PE-Vio770, 1/500, Miltenyi, Cat# 130-117-358), CD19 (APC, 1/500, Miltenyi, Cat# 130-110-250), CD4 (APC-Vio770, 1/500, Miltenyi, Cat# 130-113-223), HLA-DR (BV785, 1/20, BD Biosciences, Cat# 564041), IL-1R1 (PE, 1/80, R&D Systems, Cat# FAB269P), IL-1R2 (AF700, 1/80, R&D Systems, Cat# FAB663N), CTLA-4 (BV605, 1/40, BioLegend, Cat# 369610), and PD-1 (PE-Cy5.5, 1/320, Beckman Coulter, Cat# B36123 ). ..



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    The early life IL-17 response is dependent on IL-1 β produced by infiltrating myeloid cells. (A) Differential abundance using MiloR analysis of single cell sequencing of total CD45+ cells of weanlings and adults. (B, C) Total number of neutrophils(B) and monocytes (C) in the colon normalized to weight of the tissue of mice at indicated ages. (D, E) Total number of neutrophils (D) and monocytes (E) in the colon lamina propria of 3-week old littermate IL-17+/-and IL17-/-mice. (F) UMAP of Il1b expression in weanling and adult colon CD45+ cells. (G) Expression of Il1b in total colon of mice at indicated ages. (H, I) Representative flow plot (H) and percent (I) of pro-IL-1β+ myeloid cells in the LP of weanling and adult colons. (J) ELISA quantification of IL-1β in the supernatant of colon explants of weanling and adult mice, normalized to weight of tissue. (K, L) Percent of IL-17+ γδ T cells (K) and percent of neutrophils (L) in colonic LP of control mice and mice treated with anti-IL-1R in vivo antibody from 2-3 weeks. (M, N) Percent of IL-17+ γδ T cells (M) and percent of neutrophils (N) in colonic LP of littermate IL-1R+/-and IL-1R-/-at 3 weeks of age. (O) UMAP of type 3 immunity score and Il1r1 expression on total thymocytes from day 0 mouse (P) Percent of IL-17+ γδ T cells in 3-week-old TCRδ-/-adoptively transferred with IL-1R+/-or IL-1R-/-thymus cells. (Q) UMAP of <t>IL-1R1</t> expression in total CD45+ cells in human fetal thymus γδ T cells. (R, S ) Percent of IFNγ (R) and IL-17 (S) of Vγ9+ γδ T cells in human cord blood following stimulation with IL-1β and zoledronate. (B, C, D, E, G, I-N, P, R, and S) Each dot represents cells from an individual mouse. Data are represented as mean with SD. Graphs are pooled data from at least two independent experiments with atleast n = 3 in each experiment. (H) Contour plots are representative at least two independent experiments. Numbers indicate frequency of cells within each quadrant or box. (B, C, G) Data were analyzed using Kruskal-Wallis ANOVA test or (D, E, I-N and P) Mann-Whitney non-parametric unpaired T test (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p <0.0001).
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    il 1r1  (Biorbyt)
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    The early life IL-17 response is dependent on IL-1 β produced by infiltrating myeloid cells. (A) Differential abundance using MiloR analysis of single cell sequencing of total CD45+ cells of weanlings and adults. (B, C) Total number of neutrophils(B) and monocytes (C) in the colon normalized to weight of the tissue of mice at indicated ages. (D, E) Total number of neutrophils (D) and monocytes (E) in the colon lamina propria of 3-week old littermate IL-17+/-and IL17-/-mice. (F) UMAP of Il1b expression in weanling and adult colon CD45+ cells. (G) Expression of Il1b in total colon of mice at indicated ages. (H, I) Representative flow plot (H) and percent (I) of pro-IL-1β+ myeloid cells in the LP of weanling and adult colons. (J) ELISA quantification of IL-1β in the supernatant of colon explants of weanling and adult mice, normalized to weight of tissue. (K, L) Percent of IL-17+ γδ T cells (K) and percent of neutrophils (L) in colonic LP of control mice and mice treated with anti-IL-1R in vivo antibody from 2-3 weeks. (M, N) Percent of IL-17+ γδ T cells (M) and percent of neutrophils (N) in colonic LP of littermate IL-1R+/-and IL-1R-/-at 3 weeks of age. (O) UMAP of type 3 immunity score and Il1r1 expression on total thymocytes from day 0 mouse (P) Percent of IL-17+ γδ T cells in 3-week-old TCRδ-/-adoptively transferred with IL-1R+/-or IL-1R-/-thymus cells. (Q) UMAP of <t>IL-1R1</t> expression in total CD45+ cells in human fetal thymus γδ T cells. (R, S ) Percent of IFNγ (R) and IL-17 (S) of Vγ9+ γδ T cells in human cord blood following stimulation with IL-1β and zoledronate. (B, C, D, E, G, I-N, P, R, and S) Each dot represents cells from an individual mouse. Data are represented as mean with SD. Graphs are pooled data from at least two independent experiments with atleast n = 3 in each experiment. (H) Contour plots are representative at least two independent experiments. Numbers indicate frequency of cells within each quadrant or box. (B, C, G) Data were analyzed using Kruskal-Wallis ANOVA test or (D, E, I-N and P) Mann-Whitney non-parametric unpaired T test (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p <0.0001).
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    The early life IL-17 response is dependent on IL-1 β produced by infiltrating myeloid cells. (A) Differential abundance using MiloR analysis of single cell sequencing of total CD45+ cells of weanlings and adults. (B, C) Total number of neutrophils(B) and monocytes (C) in the colon normalized to weight of the tissue of mice at indicated ages. (D, E) Total number of neutrophils (D) and monocytes (E) in the colon lamina propria of 3-week old littermate IL-17+/-and IL17-/-mice. (F) UMAP of Il1b expression in weanling and adult colon CD45+ cells. (G) Expression of Il1b in total colon of mice at indicated ages. (H, I) Representative flow plot (H) and percent (I) of pro-IL-1β+ myeloid cells in the LP of weanling and adult colons. (J) ELISA quantification of IL-1β in the supernatant of colon explants of weanling and adult mice, normalized to weight of tissue. (K, L) Percent of IL-17+ γδ T cells (K) and percent of neutrophils (L) in colonic LP of control mice and mice treated with anti-IL-1R in vivo antibody from 2-3 weeks. (M, N) Percent of IL-17+ γδ T cells (M) and percent of neutrophils (N) in colonic LP of littermate IL-1R+/-and IL-1R-/-at 3 weeks of age. (O) UMAP of type 3 immunity score and Il1r1 expression on total thymocytes from day 0 mouse (P) Percent of IL-17+ γδ T cells in 3-week-old TCRδ-/-adoptively transferred with IL-1R+/-or IL-1R-/-thymus cells. (Q) UMAP of <t>IL-1R1</t> expression in total CD45+ cells in human fetal thymus γδ T cells. (R, S ) Percent of IFNγ (R) and IL-17 (S) of Vγ9+ γδ T cells in human cord blood following stimulation with IL-1β and zoledronate. (B, C, D, E, G, I-N, P, R, and S) Each dot represents cells from an individual mouse. Data are represented as mean with SD. Graphs are pooled data from at least two independent experiments with atleast n = 3 in each experiment. (H) Contour plots are representative at least two independent experiments. Numbers indicate frequency of cells within each quadrant or box. (B, C, G) Data were analyzed using Kruskal-Wallis ANOVA test or (D, E, I-N and P) Mann-Whitney non-parametric unpaired T test (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p <0.0001).
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    The early life IL-17 response is dependent on IL-1 β produced by infiltrating myeloid cells. (A) Differential abundance using MiloR analysis of single cell sequencing of total CD45+ cells of weanlings and adults. (B, C) Total number of neutrophils(B) and monocytes (C) in the colon normalized to weight of the tissue of mice at indicated ages. (D, E) Total number of neutrophils (D) and monocytes (E) in the colon lamina propria of 3-week old littermate IL-17+/-and IL17-/-mice. (F) UMAP of Il1b expression in weanling and adult colon CD45+ cells. (G) Expression of Il1b in total colon of mice at indicated ages. (H, I) Representative flow plot (H) and percent (I) of pro-IL-1β+ myeloid cells in the LP of weanling and adult colons. (J) ELISA quantification of IL-1β in the supernatant of colon explants of weanling and adult mice, normalized to weight of tissue. (K, L) Percent of IL-17+ γδ T cells (K) and percent of neutrophils (L) in colonic LP of control mice and mice treated with anti-IL-1R in vivo antibody from 2-3 weeks. (M, N) Percent of IL-17+ γδ T cells (M) and percent of neutrophils (N) in colonic LP of littermate IL-1R+/-and IL-1R-/-at 3 weeks of age. (O) UMAP of type 3 immunity score and Il1r1 expression on total thymocytes from day 0 mouse (P) Percent of IL-17+ γδ T cells in 3-week-old TCRδ-/-adoptively transferred with IL-1R+/-or IL-1R-/-thymus cells. (Q) UMAP of IL-1R1 expression in total CD45+ cells in human fetal thymus γδ T cells. (R, S ) Percent of IFNγ (R) and IL-17 (S) of Vγ9+ γδ T cells in human cord blood following stimulation with IL-1β and zoledronate. (B, C, D, E, G, I-N, P, R, and S) Each dot represents cells from an individual mouse. Data are represented as mean with SD. Graphs are pooled data from at least two independent experiments with atleast n = 3 in each experiment. (H) Contour plots are representative at least two independent experiments. Numbers indicate frequency of cells within each quadrant or box. (B, C, G) Data were analyzed using Kruskal-Wallis ANOVA test or (D, E, I-N and P) Mann-Whitney non-parametric unpaired T test (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p <0.0001).

    Journal: bioRxiv

    Article Title: Early life γδ T cell activation enforces intestinal barrier integrity during intergenerational C. difficile colonization

    doi: 10.64898/2026.04.28.721483

    Figure Lengend Snippet: The early life IL-17 response is dependent on IL-1 β produced by infiltrating myeloid cells. (A) Differential abundance using MiloR analysis of single cell sequencing of total CD45+ cells of weanlings and adults. (B, C) Total number of neutrophils(B) and monocytes (C) in the colon normalized to weight of the tissue of mice at indicated ages. (D, E) Total number of neutrophils (D) and monocytes (E) in the colon lamina propria of 3-week old littermate IL-17+/-and IL17-/-mice. (F) UMAP of Il1b expression in weanling and adult colon CD45+ cells. (G) Expression of Il1b in total colon of mice at indicated ages. (H, I) Representative flow plot (H) and percent (I) of pro-IL-1β+ myeloid cells in the LP of weanling and adult colons. (J) ELISA quantification of IL-1β in the supernatant of colon explants of weanling and adult mice, normalized to weight of tissue. (K, L) Percent of IL-17+ γδ T cells (K) and percent of neutrophils (L) in colonic LP of control mice and mice treated with anti-IL-1R in vivo antibody from 2-3 weeks. (M, N) Percent of IL-17+ γδ T cells (M) and percent of neutrophils (N) in colonic LP of littermate IL-1R+/-and IL-1R-/-at 3 weeks of age. (O) UMAP of type 3 immunity score and Il1r1 expression on total thymocytes from day 0 mouse (P) Percent of IL-17+ γδ T cells in 3-week-old TCRδ-/-adoptively transferred with IL-1R+/-or IL-1R-/-thymus cells. (Q) UMAP of IL-1R1 expression in total CD45+ cells in human fetal thymus γδ T cells. (R, S ) Percent of IFNγ (R) and IL-17 (S) of Vγ9+ γδ T cells in human cord blood following stimulation with IL-1β and zoledronate. (B, C, D, E, G, I-N, P, R, and S) Each dot represents cells from an individual mouse. Data are represented as mean with SD. Graphs are pooled data from at least two independent experiments with atleast n = 3 in each experiment. (H) Contour plots are representative at least two independent experiments. Numbers indicate frequency of cells within each quadrant or box. (B, C, G) Data were analyzed using Kruskal-Wallis ANOVA test or (D, E, I-N and P) Mann-Whitney non-parametric unpaired T test (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p <0.0001).

    Article Snippet: IL-1r1-/-mice (C57BL/6, No.003245) were obtained from Jackson laboratories.

    Techniques: Produced, Single Cell, Sequencing, Expressing, Enzyme-linked Immunosorbent Assay, Control, In Vivo, MANN-WHITNEY